What are (N-acetyl)hexosaminidases?
Hexosaminidases and N-acetylhexosaminidases are similar to glucosaminidases and N-acetylglucosaminidases, except that they have lower substrate specificities, removing also e.g. galactosamine or N-acetylgalactosamine residues from their respective glycosides. For chitin/chitosan research, they are equivalent to the more specific glucosaminidases and N-acetylglucosaminidases. For specific purposes, e.g. for enzymatic sequencing, it is more important to make sure that the enzymes used remove either only deacetylated or only acetylated residues which is not always the case. Some hexosaminidases/glucosaminidases may have a side activity on N-acetylglucosamine, and some N-acetylhexosaminidases/N-acetylglucosaminidases may have a side activity on glucosamine.